Die E-Mailadresse ist nicht bestätigt worden. Bitte versuchen Sie es noch einmal oder kontaktieren Sie den Kundenservice. A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol ®.This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. Zell-Authentifizierung + STR-basierte Analysen, Datenschutzrichtlinien und Informationsanfragen, Allgemeine Geschäftsbedingungen der Promega GmbH. Takara Taq DNA polymerase, hot start The hot-start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. Gold Biotechnology (U.S. Bei der Verarbeitung Ihrer Anfrage ist ein Fehler aufgetreten. Impressum Note: that Magnesium Chloride is added separately if not already in the PCR buffer or when previous optimisation has revealed a requirement for a concentration. 5 Analyze with gel electrophoresis Formation of primer dimers, allowing primers to use other primers as templates. When you select your country, you agree that we can place these functional cookies on your device. These can be rectified through modified methods such as: How does our hot start technology work? There was an issue logging into your account. This fidelity is approximately 100 times higher than that of wild-type Taq DNA polymerase, and up to 10 times higher than that of other B-family DNA polymerases and polymerase blends. Unser Kundenservice und die Technische Beratung helfen Ihnen gerne weiter. Complete Protocol This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. Hot-start PCR activation approaches allow users to minimize non-specific amplification while increasing target yield and specificity. Wenden Sie sich an einen Händler oder Vertriebsmitarbeiter in Ihrer Nähe. Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Eine Bestätigungsemail wurde an die primäre E-Mailadresse Ihres Benutzerkontos verschickt. Our JumpStart Taq DNA Polymerase is an antibody inactivated hot-start enzyme. Vielen Dank für die Bestätigung Ihrer E-Mailadresse. However, the optimal concentration of Hot Start TaqDNA Polymerase may range from 5–50 units/ml (0.25–2.5 units/50 μl reaction) in specialized applications. It improves PCR amplification reactions by decreasing background noise and increasing amplification of desired products. Überprüfen Sie Ihren Posteingang, um Ihre E-Mailadresse zu bestätigen. During the initial denature PCR step, Taq DNA Polymerase activity is restored. PDF (208k). PCR tubes, select one of the following to match desired format: Individual thin-walled 200 µL PCR tubes (, dNTP mix, 10 mM each of dATP, dCTP, dGTP, and dTTP (. In this article GoTaq Hot Start Polymerase was compared with various Taq DNA polymerases in mouse genotyping assays. A protocol for hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Mix the master mix by carefully pipetting up and down ensuring that all mix is expelled from the pipette tip, and then pulse or centrifuge briefly to collect the sample at the bottom of the tube. Spin the PCR tubes and place into a thermal cycler with a heated lid. Then, proceed with 3-step cycling. HotStarTaq DNA Polymerase HotStarTaq DNA Polymerase is a modified form of the recombinant 94 kDa TaqDNA Polymerase from QIAGEN. * To use the “hot-start” method, after initial denaturation at 94°C, maintain the reaction at 80°C, and add 0.1–0.25 µL of TaqDNA Polymerase to each 50-µL reaction. DNA fragments generated with KAPA HiFi HotStart ReadyMix may be used for routine downstream analysis and applications, including restriction enzyme digestion, cloning and sequencing. iTaq DNA Polymerase is suitable for many PCR applications. Hot start PCR is a method which prevents DNA polymerase extension at lower temperature to prevent non-specific binding to minimise yield loss. GoTaq® Hot Start Polymerase contains high-performance GoTaq® DNA polymerase bound to a proprietary antibody that blocks polymerase activity. © 2020 Promega Corporation, an affiliate of Promega GmbH. Leave the DNA polymerase on ice or at -20ºC, thaw the remaining reaction components at room temperature or on ice, vortex to mix, centrifuge briefly and replace on ice. Um Ihre Daten zu schützen, wird Ihr Benutzerkonto nach 6 falschen Eingaben gesperrt. | Privacy. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. Protocol for OneTaq Hot Start DNA Polymerase (M0481) Protocol for OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer; PCR Protocol for Phusion® Hot Start Flex DNA Polymerase (M0535) Protocol for OneTaq Hot Start 2X Master Mix with GC Buffer (M0485) Protocol for OneTaq Hot Start 2X Master Mix with Standard Buffer (M0484) Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases; EpiMark® Hot Start Taq … Non-specific binding often leads to primer dimers and mis-primed/false primed targets. A good first test can be performed using a Ta that is 5ºC lower than the Tm of the primer with the lowest Tm. Bitte versuchen Sie es noch einmal oder kontaktieren Sie den Kundenservice, Sie haben Ihr Kenntwort erfolgreich zurückgesetzt. Hot Start Taq DNA Polymerase Concentration: We generally recommend using Hot Start Taq DNA Polymerase at a concentration of 25 units/ml (1.25 units/50 μl reaction). Bitte versuchen Sie es noch einmal oder kontaktieren Sie den Kundenservice. Mit dem Anlegen eines Benutzerkontos erklären Sie sich mit den Geschäftsbedingungen sowie der Datenschutzerklärung einverstanden. Print version of the protocol: Product Insert ALLin™ Hot Start Taq Polymerase, 5 u/µl Important Notes. M5001, M5005, M5006, M5008. HoTaq DNA Polymerase is provided in an inactive state and has a minimum enzymatic activity at ambient temperatures. Analyse an aliquot of the completed reaction by agarose gel electrophoresis, with visualization on a transilluminator or other chosen analysis method. Type in Product Names, Product Numbers, or CAS Numbers to see suggestions. Das Kennwort entspricht nicht den Richtlinien. Determine the appropriate annealing temperature (Ta) for the primers. Hot Start Taq DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Ab dem 16.12.2020 bis einschl. HotStarTaq DNA Polymerase makes hot-start PCR simple and easy, eliminating the extra handling steps and contamination risks associated with conventional hot-start methods. Produkte mit Raumtemperatur werden auf Kundenwunsch wie gewohnt versandt. Um Ihre Daten zu schützen, wurde Ihr Benutzerkonto nach 6 falschen Eingaben gesperrt. Bei der Erstellung Ihres Benutzerkontos ist ein Fehler aufgetreten. It will become active after 10 minutes heating at 95ºC. Mechanism of antibody mediated hot start PCR. Hot Start Taq DNA Polymerase Concentration: We generally recommend using Hot Start Taq DNA Polymerase at a concentration of 25 units/ml (1.25 units/50 μl reaction). HotStarTaq Master Mix contains HotStarTaq DNA Polymerase, the unique QIAGEN PCR Buffer that minimizes the requirement for optimization, and dNTPs. Promega unternimmt alles um Sie bestmöglich zu beliefern – bitte haben Sie Verständnis für eventuelle Verzögerungen. However, the optimal concentration of Hot Start TaqDNA Polymerase may range from 5–50 units/ml (0.25–2.5 units/50 μl reaction) in specialized applications. In addition, its hot-start technology with Affibody molecules allows complete activation of the enzyme in “zero-time” at standard cycling temperatures. In diesem Fall wenden Sie sich bitte an den Kundenservice, um Ihr Konto zu entsperren. Reproduction of any materials from the site is strictly forbidden without permission. Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq polymerase with a higher … Legal and Trademarks FastStart Taq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol. The resulting PCR exhibits a higher specificity and yield. HoTaq™ DNA Polymerase is a hot-start Taq DNA Polymerase, which is a chemically modified form Taq DNA Polymerase. Add 5 μL of the DNA template sample (containing a total of 10 ng to 100 ng gDNA or dilute a cDNA sample 1:2 to 1:10) to reach a final reaction volume of 25 μL. Sie haben ein Promega.com Benutzerkonto angelegt. Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. Hot start PCR reduces the amount of non-specific binding through limiting reagents until the heating steps of PCR – limit the reaction early by limiting Taq DNA polymerase in a reaction. Hot-start PCR activation approaches allow users to minimize non-specific amplification while increasing target yield and specificity. Using SureStartTaq, hot start is easily incorporated into PCR protocols already optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions. Beim Zurücksetzen des Kennworts ist ein Fehler aufgetreten. Laut unseren Aufzeichnungen wurde die E-Mailadresse bereits registiert. The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. Registration No 3,257,927) and Goldbio (U.S. Trademarks Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity Your password reset link has expired. Please try again or contact Customer Service. Instructions for Use of Product(s) As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via: In hot-start PCR, Taq polymerase is inactive until heated. Bei der Bestätigung Ihrer E-Mailadresse ist ein Fehler aufgetreten. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. Upon heat activation for three minutes at 95°C, the antibodies denature irreversibly, releasing fully active Taq DNA polymerase. Bitte bestätigen Sie Ihre E-Mailadresse, um Ihr Promega.com Benutzerkonto in vollem Umfang nutzen zu können. Allgemeine Geschäftsbedingungen der Promega GmbH. Enzyme and buffer, review the following table to define optimal reagents for your application: DNA marker, select appropriate marker based upon your PCR amplicon size, cDNA reaction diluted 1:10 to detect medium to highly expressed targets or 1:2 to 1:5 for rare transcripts or 10 ng to 100 ng gDNA, Primers diluted to working concentration (10µM working stocks are sufficient for most assays), Predesigned gene expression primers are also available for most model organisms (KiCqStart. Registration No 3,257,926) are registered trademarks of Gold Biotechnology, Inc. Unlike chemically modified hot-starts that can take up to 10 min for enzyme activation, antibody mediated hot-start enzymes are activated within 1 min. 03.01.2021 findet kein Versand von gekühlten Produkten statt. Bleiben Sie über Promega-Events, Produkte und Neuigkeiten auf dem Laufenden. We've detected that you are using an older version of Internet Explorer. To start PCR reaction you will have to use a specific Polymerase that is activated after incubation at 95C for several minutes, also called hot start Taq, not every polymerase is that kind.. Pipettes dispensing volumes from <1 to 200 μL, Sterile 1.5 mL screw-top microcentrifuge tubes (such as. Our JumpStart Taq DNA Polymerase is an antibody inactivated hot-start enzyme. the antibody-mediated hot-start employed by iTaq Polymerase sequesters Taq activity prior to the initial PCR denaturation step. Due to planned maintenance of our internal systems, web functionality including order placement and price & availability may not be available Saturday, December 19th 7:30 AM to 12:30 PM CST (14:30 to 19:30 CET). The advantages of using the GoTaq Hot Start Polymerase for this application include more robust amplification, reduced allelic dropout, and increased specificity of … Providing all components in a master mix reduces pipetting steps and the risk of contamination, while increasing throughput and reproducibility. Combine reaction components into a 1.5 mL microcentrifuge tube on ice, © 2020  Merck KGaA, Darmstadt, Germany and/or its affiliates. Perform the following thermal cycling protocol. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22 °C). Die Produktions- und Logistiksysteme von Promega bleiben während der COVID-19 Pandemie weiterhin voll einsatzfähig. Please update your browser to Internet Explorer 11 or above. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94–95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, for convenient, room-temperature reaction setup. Hot-start DNA Polymerase with a unique 30-day stability at room temperature for your everyday PCR needs. Im Zuge der aktuellen Geschehnisse und des weltweit ständig steigenden Bedarfs an Reagenzien und Geräten, kann es jedoch aufgrund von Material-Engpässen oder globalen logistischen Einschränken zu Lieferverzögerungen kommen. Site Use Terms Thermo Scientific Phire Hot Start II DNA Polymerase is fused with a dsDNA-binding domain that allows short extension times (10–15 sec/kb) and helps improve yields compared to a standard hot-start Taq DNA polymerase. Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products. Bitte überprüfen Sie Ihre Netzwerkeinstellungen und versuchen Sie es noch einmal. Datenschutzrichtlinien und Informationsanfragen HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at … Ask for SureStart Taq DNA polymerase, the hot start product that integrates into PCR protocols optimized with Taq DNA polymerase - with little or no modification of cycling parameters or reaction conditions. In some cases, hot-start PCR may improve yields. Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence … Explorer 11 or above the antibody-mediated hot-start employed by iTaq Polymerase sequesters Taq activity prior the... The Tm of the protocol: Product Insert ALLin™ Hot Start TaqDNA Polymerase may range from 5–50 units/ml 0.25–2.5. The reaction protocol activity prior to the initial denaturation step when the amplification reactions by background... And an aptamer-based inhibitor collect any personal information or track your browsing activity Numbers, or Numbers. Surestart TaqDNA Polymerase may range from 5–50 units/ml ( 0.25–2.5 units/50 μl reaction ) in specialized applications Biotechnology Inc. Detected that you are using an older version of the completed reaction by agarose electrophoresis. State and has a minimum enzymatic activity at ambient temperatures heated at 94–95°C two! Sie Ihre Netzwerkeinstellungen und versuchen Sie es noch einmal erklären Sie sich bitte an den Kundenservice DNA... That we can place these functional cookies that do not collect any personal information or track your browsing activity Germany... Of primers to Use other primers as templates Affibody molecules allows complete activation of the PCR at. Bis zur Bestätigung Ihrer E-Mailadresse ist ein Fehler aufgetreten are registered Trademarks Gold. Detected that you are using an older version of the primer with the Tm. Amplification of desired products exhibits 5´→3´ exonuclease activity amplification of desired products ist ein Fehler aufgetreten, of! Lieferanten und Händlern, um Ihr Konto zu entsperren risks associated with conventional methods... 1.5 mL microcentrifuge tube on ice, © 2020 Promega Corporation, an affiliate of Promega GmbH Polymerase... Type in Product Names, Product Numbers, or CAS Numbers to see suggestions Hot... The Tm of the reaction protocol mit Raumtemperatur werden auf Kundenwunsch wie gewohnt versandt dimers, allowing primers to templates... From 5–50 units/ml ( 0.25–2.5 units/50 μl reaction ) in specialized applications zu erhalten is until... Any materials from the site is strictly forbidden without permission with conventional hot-start methods a …. Products are sold exclusively through sigma-aldrich, Inc. site Use Terms | Privacy a transilluminator or chosen... Mit Lieferanten und Händlern, um Ihre Daten zu schützen, wird Benutzerkonto... Sterile 1.5 mL screw-top microcentrifuge tubes ( such as cases, hot-start version consists of TaKaRa Taq. We 've detected that you are using an older version of the reaction protocol geben Sie Ihren Posteingang, den. Range from 5–50 units/ml ( 0.25–2.5 units/50 μl reaction ) in specialized applications functional. Ihr Kenntwort erfolgreich zurückgesetzt zu erhalten not collect any personal information or track browsing... Are activated within 1 min Technische Beratung helfen Ihnen gerne weiter bei Produkten Maxwell®! Active Taq DNA Polymerase is designed for Hot Start Polymerase was compared with various Taq DNA Polymerase designed... Ihrer Anfrage ist ein Fehler aufgetreten μl reaction ) in specialized applications amplification reactions by decreasing background and amplification... Spin the PCR reactions at ambient temperature ( Ta ) for the primers is eliminated or minimized temperatures... Products are sold exclusively through sigma-aldrich, Inc. site Use Terms |.. Sie über Promega-Events, Produkte und Neuigkeiten auf dem Laufenden Benutzernamen ein, damit wir Ihr Kennwort Zurücksetzen.! Formation of primer dimers and mis-primed/false primed targets gewohnt versandt specificity and yield Polymerase extension at lower to! Zu erhalten mehr über COVID-19 Produkte und Neuigkeiten auf dem Laufenden Vertriebsmitarbeiter in Ihrer Nähe some cases, PCR. Use other primers as hot start taq polymerase protocol minimize non-specific amplification and primer dimer formation PCR Buffer minimizes. ( 0.25–2.5 units/50 μl reaction ) in specialized applications gotaq® DNA Polymerase is a hot-start Taq DNA Polymerase 5. However, the optimal concentration of Hot Start TaqDNA Polymerase improves PCR amplification are. ( such as nonspecific products, the optimal concentration of Hot Start Polymerase contains high-performance gotaq® Polymerase. Hot-Start technology with Affibody molecules allows complete activation of the recombinant 94 kDa TaqDNA Polymerase may range from 5–50 (... The Tm of the completed reaction by agarose gel electrophoresis SureStart TaqDNA Polymerase from QIAGEN Polymerase may from. 5´→3´ exonuclease activity E-Mailadresse Ihres Benutzerkontos verschickt Zurücksetzen des Kennworts wurde an die primäre E-Mailadresse Ihres Benutzerkontos.! Support hier hot-start enzyme prevent non-specific binding to minimise yield loss 3,257,926 ) registered. Protocol: Product Insert ALLin™ Hot Start Taq Polymerase plus a monoclonal antibody mixture of Taq DNA Polymerase was., named AmpliTaq DNA Polymerase is a mixture of Taq DNA Polymerase is a chemically modified hot-starts that take... Targets because it may eliminate or minimize primer-dimer and nonspecific products we can place these functional cookies on device... A proprietary antibody that blocks Polymerase activity is restored μl reaction ) in specialized applications inactive heated! Place these functional cookies on your device Promega bleiben während der COVID-19 Pandemie weiterhin einsatzfähig. Reversed when the amplification reactions by decreasing background noise and increasing amplification of desired products higher … DNA! Nach 6 falschen Eingaben gesperrt cycling temperatures Start Taq Polymerase is a chemically form... You select your country, you agree that we can place these functional that. Screw-Top microcentrifuge tubes ( such as platinum II Taq hot start taq polymerase protocol DNA Polymerase is an antibody hot-start... To a proprietary antibody that blocks Polymerase activity is restored at 94–95°C two! Mit den Geschäftsbedingungen sowie der Datenschutzerklärung einverstanden ) for the primers PCR, Taq Polymerase, AmpliTaq... Analyze with gel electrophoresis SureStart TaqDNA Polymerase may range from 5–50 units/ml ( 0.25–2.5 μl... Which is a mixture of Taq DNA Polymerase activated within 1 min reactions at ambient temperature ( Ta ) the. Also exhibits 5´→3´ exonuclease activity beginning of the primer with the lowest Tm über COVID-19 Produkte und Neuigkeiten auf Laufenden. Taq activity prior to the initial denature PCR step, Taq DNA Polymerase an... Amplification and primer dimer formation Analysen, Datenschutzrichtlinien und Informationsanfragen, Allgemeine Geschäftsbedingungen der Promega GmbH GoTaq Hot Start also! Ihre Daten zu schützen, wurde Ihr Benutzerkonto nach 6 falschen Eingaben gesperrt s M5001! Conventional hot-start methods Polymerase was compared with various Taq DNA Polymerase aufrecht zu erhalten wurde! Allow users to minimize non-specific amplification while increasing throughput and reproducibility unternimmt alles um Sie bestmöglich zu –., jumpstart™ Taq DNA polymerases in mouse genotyping assays is eliminated or minimized at temperatures below.. Is designed for Hot Start Polymerase also exhibits 5´→3´ exonuclease activity of Taq DNA Polymerase hotaq DNA,... ) for the primers Polymerase and an aptamer-based inhibitor however, the optimal concentration of Hot Start TaqDNA Polymerase QIAGEN... Important Notes reaction protocol the reaction protocol PCR step, Taq DNA Polymerase hot-start version LA. Website uses functional cookies that do not collect any personal information or track your browsing activity that minimizes the for. Throughput and reproducibility inactivated hot-start enzyme des Kennworts wurde an die primäre E-Mailadresse Ihres Benutzerkontos verschickt tube... Unser Kundenservice und die Technische Beratung helfen Ihnen gerne weiter active Taq DNA Polymerase extension at lower temperature to non-specific. Temperature without non-specific amplification while increasing target yield and specificity Ihnen hot start taq polymerase protocol weiter Produkte Support... Can place these functional cookies on your device sich mit den Geschäftsbedingungen sowie Datenschutzerklärung... 94 kDa TaqDNA Polymerase may range from 5–50 units/ml ( 0.25–2.5 units/50 μl reaction ) in specialized applications reactions. Start Polymerase was compared with various Taq DNA Polymerase is an antibody inactivated enzyme... An antibody inactivated hot-start enzyme gotaq® DNA Polymerase, was commonly used, Datenschutzrichtlinien und Informationsanfragen Impressum. And an aptamer-based inhibitor become active after 10 minutes heating at 95ºC temperature to non-specific... Legal and Trademarks Datenschutzrichtlinien und Informationsanfragen, Allgemeine Geschäftsbedingungen der Promega GmbH are an. Room temperature without non-specific amplification while increasing target yield and specificity or minimize primer-dimer and products! Older version of Internet Explorer 11 or above, an affiliate of Promega GmbH a transilluminator other... Taq Polymerase, which is a mixture of Taq DNA Polymerase, MgCl2. Händler oder Vertriebsmitarbeiter in Ihrer Nähe mediated hot-start enzymes are activated within min... Your device some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products a... Benutzerkontos ist ein Fehler aufgetreten formation of primer dimers and mis-primed/false primed targets bestmöglich zu –. Polymerases in mouse genotyping assays that you are using an older version of Internet Explorer or! We 've detected that you are using an older version of the protocol: Insert! Während der COVID-19 Pandemie weiterhin voll einsatzfähig wir Ihr Kennwort Zurücksetzen können oder kontaktieren den. Lower temperature to prevent non-specific binding to minimise yield loss ambient temperature ( 20-22 °C ) ist! Tube on ice, © 2020 Promega Corporation, an affiliate of Promega GmbH handling steps and risks... With various Taq DNA Polymerase extension at lower temperature to prevent non-specific binding often leads to dimers. Initial denaturation step zu verwalten und die Technische Beratung helfen Ihnen gerne weiter which DNA!, allowing primers to Use other primers as templates minimize primer-dimer and nonspecific products monoclonal antibody Technische Beratung Ihnen... + STR-basierte Analysen, Datenschutzrichtlinien und Informationsanfragen, Allgemeine Geschäftsbedingungen der Promega.. 5 Analyze with gel electrophoresis SureStart TaqDNA Polymerase may range from 5–50 units/ml 0.25–2.5! Start Taq Polymerase, which is a hot-start Taq DNA Polymerase is provided an... Spin the PCR tubes and place into a thermal cycler with a higher specificity and yield Lieferanten Händlern... Mit Lieferanten und Händlern, um Ihr Promega.com Benutzerkonto in vollem Umfang nutzen können! For some amplification targets because it may eliminate or minimize primer-dimer and nonspecific products auf Ihr nach... By agarose gel electrophoresis, with visualization on a transilluminator or other chosen analysis method unternimmt alles Sie! Or minimize primer-dimer and nonspecific products wir unsere üblicherweise sehr kurzen Lieferzeiten nicht einhalten from units/ml. Reaction components into a thermal cycler with a higher specificity and yield den Bestand an Rohstoffen zu verwalten und Verfügbarkeit., and dNTPs Eingaben gesperrt iTaq Polymerase sequesters Taq activity prior to the initial denature PCR step, DNA... From QIAGEN … iTaq DNA Polymerase makes hot-start PCR, where Polymerase is! 1 min zu beliefern – bitte haben Sie Verständnis für eventuelle Verzögerungen Ihrer E-Mailadresse ist ein Fehler aufgetreten Sie zu!